DNA Sequencing and Genomics Laboratory (BIDGEN)

Overview of Services

The DNA Sequencing and Genomics Laboratory (BIDGEN) laboratory at Institute of Biotechnology was originally founded in 1990 and later in 2008 reorganized by fusing two existing laboratories into one larger unit.

Our group has two distinct functions:

1) We are a core facility providing DNA sequencing service
2) We perform research projects on several topics

• de novo genome sequencing of entire genomes
• RNA sequencing
• metagenomics and metatranscriptomics
• amplicon sequencing
• various bioinformatic analysis

Instrumentation in the lab

• Sanger Sequencing

• Sanger capillary sequencer ABI3500xL

• NGS

Since 2006 we have been utilizing the NGS (Next Generation Sequencing) approach in genomics, metagenomics and functional genomics. The constantly developing NGS technologies have enabled us to perform sequencing of eukaryotes, prokaryotes, fungi and viruses. We perform de novo assemblies and large scale population genome sequencing studies. We also do microbial diversity studies using 16S and ITS amplicon sequencing. For amplicon sequencing more than 4 000 samples can be multiplexed in one run.

We have the state of the art NGS instruments in use:

Short Reads:

1. Element Bioscience AVITI
2. Illumina MiSeq and iSeq

Long Reads:

1. PacBio Revio
   
2. Oxford Nanopore: P2 Solo
   

Single-cell transcriptomics

• 10X Genomics Chromium 

• Other instruments

• Bio-Rad Droplet Digital PCR (ddPCR)
• Roche qPCR480
• NanoString nCounter

Contacts

If you need any help using iLab - contact Pia Laine (pia.k.laine-at-helsinki.fi) or Martyn James (martyn.james-at-helsinki.fi)

Hours of operation 

Hours
Monday - Friday
8:00h - 16:00h  

Shipment and location 

Shipment/delivery address
DNA Sequencing and Genomics
Institute of Biotechnology
Viikinkaari 5D
00790 Helsinki, Finland

Location
Viikinkaari 5D
(Biocenter 2, 4th floor)
P.O.Box 56
FIN-00014 University of Helsinki 

University of Helsinki Business Identity Code: FI-03134717

Links and Resources

1. HGI BIDGEN site

Contacts

Map