Welcome!
Overview of the Services
We are one of the five nodes of the HiLIFE Genome Analysis Infrastructure (HGI) at the University of Helsinki, and offer the following genome profiling services:
Next-generation sequencing services: Services provided by FuGU include whole genome, exome, and targeted sequencing (ready-made and custom gene panels). Sequencing read length ranges from 36 to 600 bases depending on the instrument. The service includes sample QC, library preparation, sequencing, data pre-processing and analysis if requested by the customer.
Transcriptome analysis: FuGU has several options for RNA-seq sample preparation (mRNA capture, whole transcriptome sequencing, small RNA/miRNA sequencing and custom 3'UTR counting method). Transcriptome analysis includes nucleic acid QC, sample labelling, sequencing and pre-processing of the data.
NanoString nCounter targeted gene expression analysis: This biomarker analysis service is based on nCounter technology multiplexing up to 800 gene expression targets and using direct digital detection chemistry. Designed to target specific gene subsets, NanoString’s probe panels enable quantification of target RNA transcripts by a direct count of the fluorescent signals. Data analysis can be easily done by researcher using NanoString's own analysis tools.
Olink targeted biomarker protein analysis: This service is based on detecting specific protein expression by antibodies that are linked with oligos. Each protein is detected by two antibodies, which are linked with complementary oligos. Binding of the oligos can be detected and protein expression from up to 90 proteins from 88 samples is quantified using qPCR.
Nucleic acid QC: We use Qubit, Agilent TapeStation and Victor Nivo plate reader for measuring the concentration, purity and size of nucleic acids depending on the application.
Bioinformatics: We provide computational analyses for next-generation sequencing. The analysis services are categorized into basic and advanced data analysis according to the scope and objectives of the study.
Contacts
| FuGU Support: fugu-support@helsinki.fi |
|
| Hours | Location |
|
Monday - Friday 8:00h - 15:00h |
Room C504a/b Biomedicum Helsinki 1 Haartmaninkatu 8 00290 Helsinki, Finland |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Anne Mäkelä |
Lab Coordinator
|
+358-50-4420228
|
fugu-support@helsinki.fi
|
C502b
|
| Hanna Verkasalo |
NGS Service Expert
|
+358-50-4420228
|
fugu-support@helsinki.fi
|
C504b
|
| Marika Kuisma-van der Linden |
NGS Service Expert
|
+358-50-4420228
|
fugu-support@helsinki.fi
|
C504b
|
| Juho Väänänen |
Bioinformatician
|
+358-50-4420228
|
fugu-support@helsinki.fi
|
C502b
|
| Outi Monni |
Director
|
+358-50-4160736
|
outi.monni@helsinki.fi
|
